Medical Bulletin of the Ministry of Internal Affairs Medical Bulletin of the Ministry of Internal Affairs Медицинский вестник МВД 2073-8080 111343 10.52341/20738080_2025_138_5_81 Аллергология и иммунология Allergy and immunology Аллергология и иммунология ISOLATION AND CULTIVATION OF γδT-LYMPHOCYTES FROM TISSUES AND ORGANS OF HUMANS AND ANIMALS: PRINCIPAL APPROACHES. REVIEW OF LITERATURE ВЫДЕЛЕНИЕ И КУЛЬТИВИРОВАНИЕ γδΤ-ЛИМФОЦИТОВ ИЗ ТКАНЕЙ И ОРГАНОВ ЧЕЛОВЕКА И ЖИВОТНЫХ: ОСНОВНЫЕ ПОДХОДЫ. ОБЗОР ЛИТЕРАТУРЫ Свитич Оксана Анатольевна Svitich Oksana A. svitichoa@yandex.ru

доктор медицинских наук;

doctor of medical sciences;

 КАЛИНИЧЕНКО Е. О. KALINIChENKO E. O. eugeniuscallinicus@yandex.ru

кандидат медицинских наук;

candidate of medical sciences;

 БИШЕВА И. В. BIShEVA I. V. ibisheva@yandex.ru СОРОКИНА Е. В. SOROKINA E. V. sorokina-cathrine@yandex.ru

доктор медицинских наук;

doctor of medical sciences;

 Первый Московский государственный медицинский университет им. И. М. Сеченова Москва Россия First Moscow State Medical University named after I.M. Sechenov Moscow Russian Federation ФГБНУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова» Россия Federal State Budgetary Scientific Institution "I.I. Mechnikov Research Institute of Vaccines and Sera" Russian Federation ФГБНУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова» Россия I.I. Mechnikov Research Institute of Vaccines and Serums Russian Federation ФГБНУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова» Москва Россия I.I. Mechnikov Scientific Research Institute of Vaccines and Serums Moscow Russian Federation ФГБУ «Федеральный научно- клинический центр специализированных видов медицинской помощи и медицинских технологий Федерального медико-биологического агентства» Россия Federal State Budgetary Institution "Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies of the Federal Medical and Biological Agency" Russian Federation 30 12 2025 30 12 2025 2025 5 81 84 30 12 2025 https://vestnikmvd.editorum.ru/en/nauka/article/111343/view

Способность γδТ-лимфоцитов расСВИТИЧ О.А., академик РАН, д.м.н., профессор РАН, директор ФГБНУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова», пропознавать антигены независимо от главного комплекса гистосовместимости, а также продуцировать цитокины и цитотоксические молекулы делает их перспективными объектами для иммунотерапии. Тканерезидентные γδТ-клетки играют важную роль в иммунном надзоре, поддержании гомеостаза и патогенезе различных заболеваний, включая инфекционные, онкологические и аутоиммунные процессы. Однако методы выделения и культивирования γδТ-лимфоцитов остаются сложными и недостаточно стандартизированными, особенно для тканевых источников. В данном обзоре рассматриваются современные подходы к получению этих клеток из различных тканей человека (биоптатов, операционного материала) и животных (преимущественно мышей).

The γδT-lymphocytes’ capacity to recognize antigens regardless of the major histocompatibility complex and to produce cytokines and cytotoxic molecules makes them promising objects for immunotherapy. The tissue-resident γδT-cells play an important role in immune surveillance, maintaining homeostasis and pathogenesis of various diseases including infectious, oncological and autoimmune processes. However the methods for isolating and cultivating γδT-lymphocytes remain complicated and poorly standardized, especially for those derived from tissue sources. This review focuses on the modern approaches to isolation of these cells from various tissues of humans (biopsies, surgical materials) and animals (mainly mice).

 γδΤ-лимфоциты тканерезидентные лимфоциты выделение клеток культура лимфоцитов γδΤ-lymphocytes tissue-resident lymphocytes isolating cells culture of lymphocytes

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